Very first, rather than the kit's EDTA-that contains elution buffer, 10 mM Tris, pH7-eight is Ordinarily used for the final elution, specifically for samples that happen to be to get sequenced with PacBio chemistry, considering the fact that even small quantities of EDTA can impede downstream processes included in sequencing https://www.facebook.com/NewTechFind/posts/pfbid0C5fmSXJrapHFYG52mcPLSN2nTbXxqHYvipy9fSmo2CxcnbiVbpEedVhmhkpwRBjyl